An overhead shot of a sleazy gambling den gives way to a guy stuffing his winnings into a briefcase. A gal winks to another before going to assist Mr. Lucky. Cut to an all female gang wearing hot pants and riding trail bikes. Swinging on a light fitting our heroine drops the guy with a scissors hold before shooting to kill and calling in her buddies. Driving their red 4WD with white skulls on the bonnet through the front doors, the girls go gun crazy and escape with the cash. A survivor reports back to the big boss saying they were raided by hundreds. He's promptly told to kill all the women and dump their bodies in the river. He's also warned that Mr. Tiger will be informed.

Out on the road we're treated to close ups of the pack in their all-purpose uniforms of short shorts, tight tops, and go-go boots. A violent flashback from the femme leader reveals the reason behind her current craving for violent retribution.

Back to the war where the gals make a night raid involving bikes, jumps, explosions and one hell of a poorly conceived/executed gag with a bike on wires. A false floor traps several gals who immediately become subjects for the secret lab where they plan to corner the world market in aphrodisiacs. A bad cover version of Nights In White Satin jumps out of the busy music soundtrack. A lab demo goes down (literally). A guy comes (off screen) and the gal spits white fluid into Mr. Tiger's face. Thrown into the dungeon she's let loose in a world of torture that involves being trapped in a sack with an angry possum and getting punched and kicked onto barbed wire while hanging from a rope.

Basically a women in prison flick from here on in, Virgins From Hell then racks up the exploitation points including but not limited to spastic dancing (for Mr. Tiger), exploding face bubbles, a bullet sucking reptile, a face sucking leech attack, a croc attack, intermittent soft core nudity, head banging, Mr. T in a poncho, a gal being roasted on a spit, the obviously fake red painted rocks on the aforementioned barbecue and choice dialogue like, "I'll get you, you stupid sluts". Suffice to say it all ends in flames with Larry the ass-kicking pharmacist furiously exchanging enzymes as the remaining team of gal pals look on.